Prof. Bekir SALİH - Eurasia Biochemical Approaches & Technologies Congress (EBAT)

Prof. Bekir SALİH



Prof. Bekir SALİH

Hacettepe University


 Present Occupation:    Hacettepe University, Chemistry Department,

1990-Ph.D.:    in Chemistry, Hacettepe University, Turkey.




1. Research Associate, Catholic University, Nijmegen, The Netherlands, 1983 (5 months).

2. ICSC Wilhelm Simon Visiting Professor, Swiss Federal Institute of Technology (ETH), Zürich, Switzerland, 1998 (13 Months).

3. Visiting Professor, Konstanz University, Konstanz, Germany, 2002 (3 months).

4. Visiting Professor, Eötvös Loránd University University, Budapest, Hungary, 2005 (3 months).



Mass Spectrometry, Chromatographic and Electrophoretic Techniques, Non-covalent Complexes, Proteomics, Metabolomics, Molecular Switches, Sol-Gels and post-translational modifications, therapeutic drugs and aptamers.



He directed 16 PhD and 28 MSc thesis. He is the author of 8 books.

He published more than 230 publications, presented 64 presentations in the international meetings. He has more than 6500 citations and his h index is 45.

He was in the organizing member of 4 international conferences.

He is the instrumental analysis expert of the International Atomic Energy Agency since 2005 in the southern part of the world.

He was awarded Hacettepe University Science award in 2007.

He is also the member of Turkish Academy of Science. Since 2020 he has been a member of the council of Turkish Academy of Science

He used to be the head of Turkish Doping Control Center. During that period the center was accredited by Bekir Salih and his team.  

He used to be the Dean of Science Faculty between 2013-2015, at Hacettepe University.



He directed 52 projects having about 8.000.000 US $ budget.



  1. Atakay, M., Aksakal, F., Bozkaya, U., Salih, B., & Wesdemiotis, C. (2020). Conformational Characterization of Polyelectrolyte Oligomers and Their Noncovalent Complexes Using Ion Mobility-Mass Spectrometry. Journal of the American Society for Mass Spectrometry.
  2. Ülkü, Ö., Atakay, M., Kohneshahri, M. Y., Uzun, C., & Salih, B. (2020). Site-Specific characterization of peptide-polymer conjugates in various stoichiometries by MALDI-Tandem mass spectrometry. Microchemical Journal, 152, 104467.
  3. Bayramoglu, G., Kayili, H. M., Oztekin, M., Salih, B., & Arica, M. Y. (2020). Hydrophilic spacer-arm containing magnetic nanoparticles for immobilization of proteinase K: Employment for speciation of proteins for mass spectrometry-based analysis. Talanta, 206, 120218.
  4. Koçak, Ö. F., Kayili, H. M., Albayrak, M., Yaman, M. E., Kadıoğlu, Y., & Salih, B. (2019). N-glycan profiling of papillary thyroid carcinoma tissues by MALDI-TOF-MS. Analytical biochemistry, 584, 113389.
  5. Bayramoglu, G., Kayili, H. M., Oztekin, M., Salih, B., & Arica, M. Y. (2020). Hydrophilic spacer-arm containing magnetic nanoparticles for immobilization of proteinase K: Employment for speciation of proteins for mass spectrometry-based analysis. Talanta, 206, 120218.
  6. BAYRAMOGLU, G. Salih, B.; Akbulut, A.; Arica, M.Y. Biodegradation of Cibacron Blue 3GA by insolubilized laccase and identification of enzymatic byproduct using MALDI-ToF-MS: Toxicity assessment studies by Daphnia magna and Chlorella vulgaris, ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 170, 453-460, 2019.
  7. WEI, B.Q.; Gerislioglu, S.; Atakay, M.; Salih, B.; Wesdemiotis, C. Characterization of supramolecular peptide-polymer bioconjugates using multistage tandem mass spectrometry. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY. 436 130-136, 2019.
  8. BAYRAMOGLU, G.; Ozalp, V.C. ; Oztekin, M. ; Guler, U.;Salih, B.; Arica, M.Y. Design of an aptamer-based magnetic adsorbent and biosensor systems for selective and sensitive separation and detection of thrombin. TALANTA. 191 59-66, 2019.
  9. Wei, B., Gerislioglu, S., Atakay, M., Salih, B., & Wesdemiotis, C. (2019). Characterization of supramolecular peptide-polymer bioconjugates using multistage tandem mass spectrometry. International Journal of Mass Spectrometry, 436, 130-136.
  10. Stavenhagen, K., Kayili, H. M., Holst, S., Koeleman, C. A., Engel, R., Wouters, D., Zeerleder, S., Salih B., & Wuhrer, M. (2018). N-and O-glycosylation analysis of human C1-inhibitor reveals extensive mucin-type O-glycosylation. Molecular & Cellular Proteomics, 17(6), 1225-1238.
  11. Kayili, H. M., Barlas, N., Atakay, M., & Salih, B. (2018). Fast purification of glycans and glycopeptides using silk-packed micropipette tip for matrix-assisted laser desorption/ionization-mass spectrometry and high-performance liquid chromatography-fluorescence detection analysis. Microchemical Journal, 139, 492-499.
  12. Atakay, M., Çelikbıçak, Ö., & Salih, B. (2018). Use of Sulfonates as Desorption Agents for Phosphopeptide Elution from an Anion-exchange Material. Analytical Letters, 51(5), 760-772.
  13. Salimi K, Usta DD, Celikbicak O, Pinar A, Salih B, Tuncel A. Ti(IV) carrying polydopamine-coated, monodisperse-porous SiO2 microspheres with stable magnetic properties for highly selective enrichment of phosphopeptides. COLLOIDS AND SURFACES B-BIOINTERFACES. 2017 (153) 280-290.
  14. Akillioglu HG, Celikbicak O, Salih B, Gokmen V. Monitoring protein glycation by electrospray ionization (ESI) quadrupole time-of-flight (Q-TOF) mass spectrometer. FOOD CHEMISTRY. 2017 (217) 65-73.
  15. Kayili HM, Salih B. Fast and efficient proteolysis by reusable pepsin-encapsulated magnetic sol-gel material for mass spectrometry-based proteomics applications. Talanta.2016 (155) 78-86.
  16. Bayramoglu G, Arica MY, Liman G, Celikbicak O, Salih B. Removal of bisphenol A from aqueous medium using molecularly surface imprinted microbeads. CHEMOSPHERE. 2016 (150) 275-284.
  17. Bayramoglu, Gulay; Ozalp, V. Cengiz; Yilmaz, Meltem; et al. Lysozyme specific aptamer immobilized MCM-41 silicate for single-step purification and quartz crystal microbalance (QCM)-based determination of lysozyme from chicken egg white. MICROPOROUS AND MESOPOROUS MATERIALS. 2015, 207: 95-104.
  18. Yazici, Aysegul; Sarcicek, Bakiye; Altindal, Ahmet; et al. Synthesis and characterization, electrical and gas sensing properties of N, N '-bis(salicylidene)-1,2-phenylendiamine substituted novel mono and ball-type metallo phthalocyanines. INORGANICA CHIMICA ACTA. 2015, 428: 83-92   Published: MAR 24 2015.
  19. Kavruk, M.; Celikbicak, O.; Ozalp, V. C.; et al. Antibiotic loaded nanocapsules functionalized with aptamer gates for targeted destruction of pathogens . CHEMICAL COMMUNICATIONS. 2015, 51(40): 8492-8495   Published: 2015.
  20. Kaki, Esra; Altindal, Ahmet; Salih, Bekir; et al. Synthesis, characterization and gas sensing properties of novel homo and hetero dinuclear ball-type phthalocyanines. DALTON TRANSACTIONS. 2015, 44(17): 8293-8299.
  21. Pekdemir, Fatih; Kurnali, Sertan; Sengul, Abdurrahman; et al. A conformationally stressed novel ball-type perylenediimide appended zinc(II) phthalocyanine hybrid: spectroelectrochemical, electrocolorimetric and photovoltaic properties  DALTON TRANSACTIONS. 2015, 44(1): 158-166.
  22. Celikbicak, Omur; Salih, Bekir; Wesdemiotis, Chrys. Strong ionic interactions in noncovalent complexes between poly(ethylene imine), a cationic electrolyte, and Cibacron Blue, a nucleotide mimic - implications for oligonucleotide vectors . 2014, 49(7): 597-607.
  23. Bayramoglu, Gulay; Celikbicak, Omur; Arica, M. Yakup; et al.Trypsin Immobilized on Magnetic Beads via Click Chemistry: Fast Proteolysis of Proteins in a Microbioreactor for MALDI-ToF-MS Peptide Analysis.  INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH. 2014, 53(12): 4554-4564.
  24. Kaki, Esra; Ozkaya, Ali Riza; Altindal, Ahmet; et al.Synthesis, characterization, electrochemistry and VOC sensing properties of novel metallophthalocyanines with four cyclohexyl-phenoxyphthalonitrile groups .SENSORS AND ACTUATORS B-CHEMICAL. 2013, 188: 1033-1042.
  25. Celikbicak, Omur; Kaynar, Gizem; Atakay, Mehmet; et alSpecific enrichment and direct detection of phosphopeptides on insoluble transition metal oxide particles in matrix-assisted laser desorption/ionization mass spectrometry applications. . Eur. J. Mass Spect. 2013, 19(3): 151-162.  
  26. Ozer LM, Ozer M, Altindal A, Ozkaya AR, Salih B, Bekaroglu O. Synthesis, characterization, OFET and electrochemical properties of novel dimeric metallophthalocyanines. Dalton Trans. 2013 May 14;42(18):6633-44.
  27. Çelikbıçak Ö, Atakay M, Güler Ü, Salih B. A Novel Tantalum-based Sol-gel Packed Microextraction Syringe for Highly Specific Enrichment of Phosphopeptides in MALDI-MS Applications, Analyst, 2013 (138):4403-4410.
  28. Salan U, Altindal A, Ozkaya AR, Salih B, Bekaroglu O. Photovoltaic and electrocatalytic properties of novel ball-type phthalocyanines bridged with four dicumarol. Dalton Transactions. 2012;41(17):5177-87.
  29. Celikbicak O, Demirel G, Piskin E, Salih B. Small molecule analysis using laser desorption/ionization mass spectrometry on nano-coated silicon with self-assembled monolayers. Analytica Chimica Acta. 2012 Jun; 729:54-61.
  30. Atakay M, Celikbicak O, Salih B. Amine-Functionalized Sol-Gel-Based Lab-in-a-Pipet-Tip Approach for the Fast Enrichment and Specific Purification of Phosphopeptides in MALDI-MS Applications. Analytical Chemistry. 2012 Mar;84(6):2713-20.
  31. Kayaoglu G, Omurlu H, Akca G, Gurel M, Gencay O, Sorkun K, Salih B. Antibacterial Activity of Propolis versus Conventional Endodontic Disinfectants against Enterococcus faecalis in Infected Dentinal Tubules. Journal of Endodontics. 2011 Mar;37(3):376-81.
  32. Elmali D, Altindal A, Ozkaya AR, Salih B, Bekaroglu O. Synthesis and characterization, novel across adjacent ring formed phthalocyanine. Dalton Transactions. 2011;40(3):651-60.
  33. Odabas Z, Altindal A, Ozkaya AR, Salih B, Bekaroglu O. Novel ball-type homo- and hetero-dinuclear phthalocyanines with four 1,1 '-methylenedinaphthalen-2-ol bridges: Synthesis and characterization, electrical and gas sensing properties and electrocatalytic performance towards oxygen reduction. Sensors and Actuators B-Chemical. 2010 Mar;145(1):355-66. 
  34. Ceyhan T, Altindal A, Ozkaya AR, Salih B, Bekaroglu O. Novel ball-type four dithioerythritol bridged metallophthalocyanines and their water-soluble derivatives: Synthesis and characterization, and electrochemical, electrocatalytic, electrical and gas sensing properties. Dalton Transactions. 2010;39(41):9801-14.
  35. Salih B, Sipahi T, Donmez EO. Ancient nigella seeds from Boyali Hoyuk in north-central Türkiye. Journal of Ethnopharmacology. 2009 Jul;124(3):416-20.
  36. Girgin G, Baydar T, Ledochowski M, Schennach H, Bolukbasi DN, Sorkun K, Salih B, Sahin G, Fuchs D. Immunomodulatory effects of Turkish propolis: Changes in neopterin release and tryptophan degradation. Immunobiology. 2009 Feb;214(2):129-34.
  37. Ceyhan T, Altindal A, Ozkaya AR, Salih B, Bekaroglu O. Synthesis, characterization, and electrocatalytic and electrical properties of novel ball-type four cyclopentyldisilanoxy-POSS bridged metallophthalocyanines. Dalton Transactions. 2009 (46):10318-29.
  38. Ceyhan T, Yuksek M, Yaghoglu HG, Salih B, Erbil MK, Elmali A, Bekaroglu O. Synthesis, characterization and nonlinear absorption of novel octakis-POSS substituted metallophthalocyanines and strong optical limiting property of CuPc. Dalton Transactions. 2008 (18):2407-13.
  39. Yarasir MN, Koca A, Kandaz M, Salih B. Voltammetry and spectroelectrochemical behavior of a novel octapropylporphyrazinato Lead(II) complex. Journal of Physical Chemistry C. 2007 Nov;111(44):16558-63. 
  40. Tuncel D, Ozsar O, Tiftik HB, Salih B. Molecular switch based on a cucurbit 6 uril containing bistable 3 rotaxane. Chemical Communications. 2007 (13):1369-71.
  41. Salih B, Celikbicak O, Doker S, Dogan M. Matrix elimination method for the determination of precious metals in ores using electrothermal atomic absorption spectrometry. Analytica Chimica Acta. 2007 Mar;587(2):272-80. 
  42. Koru O, Toksoy F, Acike CH, Tunca YM, Baysallar M, Guclu AU, Akca E, Ozkok A, Sorkun K, Tanyuksel M, Salih B. In vitro antimicrobial activity of propolis samples from different geographical origins against certain oral pathogens. Anaerobe. 2007 Jun-Aug;13(3-4):140-5.
  43. Erdag B, Balcioglu KB, Kumbasar A, Celikbicak O, Zeder-Lutz G, Altschuh D, Salih B, Baysal K. Novel short peptides isolated from phage display library inhibit vascular endothelial growth factor activity. Molecular Biotechnology. 2007 Jan;35(1):51-63.
  44. Tuncel D, Tiftik HB, Salih B. PH-Responsive polypseudorotaxane synthesized through cucurbit 6 uril catalyzed 1,3-dipolar cycloaddition. Journal of Materials Chemistry. 2006;16(32):3291-6.
  45. Kavakli C, Malci S, Tuncel SA, Salih B. Selective adsorption and recovery of precious metal ions from geological samples by 1,5,9,13-tetrathiacyclohexadecane-3,11-diol anchored poly(p-CMS-DVB) microbeads. Reactive & Functional Polymers. 2006 Feb;66(2):275-85.
  46. Ceyhan T, Altindal A, Ozkaya AR, Erbil MK, Salih B, Bekaroglu Z. Synthesis, characterization, and electrical, electrochemical and gas sensing properties of a novel ball-type four t-butylcalix 4 arene bridged binuclear zinc(II) phthalocyanine. Chemical Communications. 2006 Jan (3):320-2.  
  47. Pekel N, Salih B, Guven G. Metal ion promoted hydrogels for bovine serum albumin adsorption: Cu(II) and Co(II) chelated poly (N-vinylimidazole)-maleic acid. Colloids and Surfaces B-Biointerfaces. 2005 May;42(2):89-96.
  48. Kilic AG, Malci S, Celikbicak O, Sahiner N, Salih B. Gold recovery onto poly(acrylamide-allylthiourea) hydrogels synthesized by treating with gamma radiation. Analytica Chimica Acta. 2005 Aug;547(1):18-25.
  49. pre-concentration of Pt(II) and Pd(II). Analytica Chimica Acta. 2005 Nov;553(1-2):73-82.
  50. Abay I, Denizii A, Kin EB, Salih B. Removal and pre-concentration of phenolic species onto beta-cyclodextrin modified poly(hydroxyethylmethacrylate-ethyleneglycoldimethacrylate) microbeads. Chemosphere. 2005 Dec;61(9):1263-72.
  51. Pekel N, Salih B, Guven O. Activity studies of glucose oxidase immobilized onto poly(N-vinylimidazole) and metal ion-chelated poly(N-vinylimidazole) hydrogels. Journal of Molecular Catalysis B-Enzymatic. 2003 Feb;21(4-6):273-82.
  52. Aktas N, Sahiner N, Kantoglu O, Salih B, Tanyolac A. Biosynthesis and characterization of laccase catalyzed poly(catechol). Journal of Polymers and the Environment. 2003 Jul;11(3):123-8.
  53. Kolankaya D, Selmanoglu G, Sorkun K, Salih B. Protective effects of Turkish propolis on alcohol-induced serum lipid changes and liver injury in male rats. Food Chemistry. 2002 Aug;78(2):213-7.
  54. Kavakli C, Ozvatan N, Tuncel SA, Salih B. 1,4,8,11-tetraazacyclotetradecane bound to poly(p-chloromethylstyrene-ethylene glycol dimethacrylate) microbeads for selective gold uptake. Analytica Chimica Acta. 2002 Aug;464(2):313-22.
  55. Lehmann E, Salih B, Gomez-Lopez M, Diederich F, Zenobi R. Do matrix-assisted laser desorption/ionization mass spectra reflect solution-phase formation of cyclodextrin inclusion complexes? Analyst. 2000;125(5):849-54.
  56. Salih, B; Zenobi, R ,.MALDI mass spectrometry of dye-peptide and dye-protein complexes. Anal. Chem. 1998 (70): 1536-1543.
  57. Seker S, Beyenal H, Salih B, Tanyolac A. Multi-substrate growth kinetics of Pseudomonas putida for phenol removal. Applied Microbiology and Biotechnology. 1997 May;47(5):610-4.
  58. Denizli A, Salih B, Piskin E. New sorbents for removal of heavy metal ions: Diamine-glow-discharge treated polyhydroxyethylmethacrylate microspheres. Journal of Chromatography A. 1997 Jun;773(1-2):169-78.
  59. Beyenal H, Seker S, Tanyolac A, Salih B. Diffusion coefficients of phenol and oxygen in a biofilm of Pseudomonas putida. Aiche Journal. 1997 Jan;43(1):243-50.
  60. Arica MY, Denizli A, Salih B, Piskin E, Hasirci V. Catalase adsorption onto cibacron blue F3GA and Fe(III)derivatized poly(hydroxyethyl methacrylate) membranes and application to a continuous system. Journal of Membrane Science. 1997 Jun;129(1):65-76. 

"The Power of Ion Mobility-Mass Spectrometry for Structural Characterization and Conformational Analyses of Therapeutic Drugs" 

Therapeutic drug (mAb) is the largest class of biomolecule-based drugs in the pharmaceutical industry. The production and analysis of these drugs are quite difficult due to their complex chemical structures. They have many variants of the same protein due to variable post-translational modifications depends to the production media. Mainly separation techniques coupled mass spectrometry and direct infusion mass spectrometric techniques were used for the detailed therapeutic drug analysis in their intact form and after reduction process using various enzymes and reducing agents. Following these analyses techniques tandem mass spectrometry was used due to analyze the posttranslational modifications and the amino acid sequences changing during the drug productions. In addition, conformational analyzes of therapeutic drugs are carried out using ion mobility technique combined mass spectrometry (IM-MS) based on the separation of ions in the gas phase according to their collision cross-section (CCS) values as a size parameter1. In this way, fast and straightforward configured systems were applied to perform full therapeutic drug analyses in their intact form without using any additional separation techniques prior to mass spectrometric analysis. Using IM-MS exactly same masses but different configurations of biomolecules and their fragments could be differentiated from each other effectively. In this study some denaturing agent and specific enzymes were used to define the therapeutic drug fragments using mainly ion mobility-mass spectrometric techniques. Immune globulin G and Bevacizumab therapeutic drug was used as model drug and ion mobility-mass spectrometric technique was used for analysis intact form also digested form of therapeutic drugs. It could be noted that IM-MS had capability to obtain advanced analysis in a short time for therapeutic drugs.